Trypsin 0.05% – EDTA 0.53 mM (1X)
Atlanta Biologicals provides the most commonly used trypsin-based dissociation reagents for cell isolation, allowing for a simple and reliable dissociation process in cell culture. Each product is carefully manufactured to minimize loss of enzyme activity from denaturation and autodegradation, and quality controlled to ensure consistency and reliability.
Trypsin 0.05% is the trypsin solution of choice for more sensitive cell lines. EDTA (Ethylenediaminetetraacetic acid), a chelating agent that neutralizes calcium and magnesium ions, is added to increase the enzymatic activity on the cells and to facilitate release of individual cells from the culture vessel. Calcium and magnesium ions enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.
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In cell culture research and cell biology studies enzymes are widely used to dissociate tissues and cell monolayers. The goal of cell isolation is to maximize the yield of functionally viable, dissociated cells. Despite the widespread use of enzymes for cell isolation applications over the years, very little is known about their mechanisms of action in cell dissociation and harvesting. Selection of the trypsin enzyme preparation of choice is dependent upon the type cells in culture.
Trypsin solutions are enzyme preparations derived from porcine pancreas, generally used for the isolation and dissociation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary for the successful isolation of cells depends primarily on the cell type and the age of the culture.
- Contains 0.5 gm/L of trypsin (1:250) and 0.2 gm/L EDTA.4Na in Hanks’ Balanced Salt Solution without calcium and magnesium salts
- Form: Liquid
- Product Size: 100 ml
- Processing: 0.2μm filtered
- Sterility Testing: Pass
- Quality Testing: Pass
- Suitability: Laboratory research applications
- Shipping conditions: On dry ice
- Storage conditions: -5 to -20°C, protected from light