GlutaminePlus – 200mM (100X)
$29.90
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GlutaminePlus is an alternative to L-glutamine that can improve cell viability and growth. It is used as an effective substitute for L-glutamine in cell culture media.
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Description
Normal L-glutamine has a relatively short half-life in solution, showing a temperature-dependent loss of product with time. L-glutamine degrades through an intramolecular cyclization reaction forming, as by-products, pyrrolidone carbonic acid and ammonia in equimolar concentrations. The rate of breakdown increases with increasing temperature and in the presence of phosphate or bicarbonate.
Dipeptide derivatives of L-glutamine prevent the intramolecular cyclization reaction associated with solutions of L-glutamine. These derivatives are therefore stable in solution and allow the formulation of cell culture media containing L-glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can even be autoclaved without appreciable degradation of the product (30 minutes at 121°C results in approximately <5% loss of the product).
The dipeptide derivatives are metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.
Specifications
- Contains 200mM GlutaminePlus in 0.85% saline
- Recommended concentration: 10 ml/L
- Form: Liquid
- Product Size: 100 ml
- Processing: 0.2μm filtered
- Sterility Testing: Pass
- Quality Testing: Pass
- Suitability: Laboratory research applications
- Shipping conditions: Ambient
- Storage conditions: 8 to -20°C, protected from light
Technical Information
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