F-12, 25mM HEPES, GlutaminePlus, no phenol red


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Ham’s Nutrient Mixture F-12, liquid
with 25mM HEPES buffer
with GlutaminePlus
without phenol red

Catalog #: M156
SizePriceCatalog #Quantity
500 ml$45.00M15650
Minimum order quantity: 12

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Ham’s Nutrient Mixtures, originally designed to support the serum-free growth of Chinese Hamster Ovary (CHO) cells, are now used with serum supplementation for a variety of cell types. Ham’s Nutrient Mixture F-12 features higher concentrations of some components, as compared to Ham’s F-10. Atlanta Biologicals offers a selection of Ham’s F-12 modifications, including versions with HEPES buffer for a more effective buffering capacity.

Ham’s F-12 Nutrient Mixture (F-12)


  • GlutaminePlus


  • Phenol Red

Ham’s F-12 typically requires supplementation with serum, mostly 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.


  • Form: Liquid
  • Product Size: 500 ml
  • Sterility Testing: Pass
  • Suitability: Laboratory research applications
  • Shipping conditions: Ambient
  • Storage conditions: 2-8°C, protected from light