Antibiotics and Antimycotics
Antibiotic mixtures are widely used for the prophylactic control or the elimination of a broad spectrum of microbial contaminants from mammalian and insect cell cultures. However, antibiotic mixtures may exert cytotoxic effects in specific cell culture systems at certain concentrations. Prescreening is recommended to determine the optimal dilution of an antibiotic or antimycotic solution. The use of antibiotics should be discontinued periodically to avoid the emergence of antibiotic-resistant organisms and the masking of cryptic microbial contaminants in culture.
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