RPMI 1640 was developed by Moore and his co-workers as a modification of McCoy’s 5A medium while at Roswell Park Memorial Institute in 1966. Originally developed for human leukemic cells in monolayer or suspension cultures, it has since been shown to support a broad spectrum of anchorage-dependent and suspension mammalian cells. It is also widely used in hybridoma fusion protocols and to support the growth of hybrid cells.

This enriched medium is available with or without L-glutamine, with our stable L-glutamine substitute, and with HEPES buffer.