Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and F-12. This mixture was found to support the growth of a wide range of mammalian cells under serum-free conditions when supplemented with hormones, growth factors and additional nutrients. HEPES buffer at a final concentration of 15mM is included to compensate for the loss of buffering capacity resulting from the omission of serum.

DMEM/F-12 is also available without L-glutamine and with our stable L-glutamine substitute.