DMEM/F-12

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and F-12. This mixture was found to support the growth of a wide range of mammalian cells under serum-free conditions when supplemented with hormones, growth factors and additional nutrients. HEPES buffer at a final concentration of 15mM is included to compensate for the loss of buffering capacity resulting from the omission of serum.

DMEM/F-12 is also available without L-glutamine and with our stable L-glutamine substitute.

Showing all 12 results

    Select your DMEM/F-12 medium:

    Catalog No with L-glutamine with Glutamine Plus* with HEPES
    15mM
    with phenol red
    M23050
    M23550
    M23350
    M23750
    M23250
    M23850
    M23150
    M23650
    M23950
    M33050
    M23450
    M33150

                                                                                               *Stable L-Glutamine

    Catalog No Product Name
    M23750 DMEM/F-12, no L-glutamine, no phenol red
    M23350 DMEM/F-12, no glutamine
    M23650 DMEM/F-12, L-glutamine, no phenol red
    M23150 DMEM/F-12, L-glutamine
    M33150 DMEM/F-12, GlutaminePlus, no phenol red
    M23450 DMEM/F-12, GlutaminePlus
    M23550 DMEM/F-12, 15mM HEPES, no glutamine, no phenol red
    M23050 DMEM/F-12, 15mM HEPES, no glutamine
    M23850 DMEM/F-12, 15mM HEPES, L-glutamine, no phenol red
    M23250 DMEM/F-12, 15mM HEPES, L-glutamine
    M33050 DMEM/F-12, 15mM HEPES, GlutaminePlus, no phenol red
    M23950 DMEM/F-12, 15mM HEPES, GlutaminePlus