Ham’s Nutrient Mixture F-12 (F-12) was originally formulated for the serum-free growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L-cells. It now is widely used with whole or dialyzed serum, or in combination with hormones and other supplements for serum-free cultures. F-12 supports the growth of a broad spectrum of mammalian and hybridoma cells.

Ham’s F-12 is also available with HEPES buffer, providing a more effective buffering capacity in the optimum pH range of 7.2 to 7.4.