Dulbecco’s Modified Eagle’s Medium (DMEM) is one of many modifications of Eagle’s BME and possibly the most widely used. DMEM was originally formulated by Renato Dulbecco to support the growth of non-transformed mouse and chicken cells. In comparison with BME, it contains a four-fold higher concentration of amino acids, vitamins, and additional components. It is now available in various modifications of the original formulation. DMEM and its modifications, typically used with a serum supplement, support the growth of a broad spectrum of mammalian cells.

DMEM is available in high glucose and low glucose formulations, with different combinations of L-glutamine and sodium pyruvate. Additionally, these formulations are also offered with further modifications.

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